MVCC Concentration Solution and Dilution Lab Report

Help me study for my Biology class. I’m stuck and don’t understand.

Procedure

  1. Gather the volumetric flask, distilled water, graduated pipet, pipet bulb, sugar, scale, glass beaker, cup (plastic or drinking), scissors, and a sheet of white paper.
  2. Tare the digital scale by pressing the Φ/T button so that the scale reads 0.0 g.
  3. Remove the stopper for the volumetric flask from the top of the flask.
  4. Place the dry volumetric flask on the scale to determine its mass. Record the mass of the volumetric flask in Data Table 8.

Note: You will use the mass of the dry volumetric flask throughout the experiment.

  1. Cut a small square of white paper, fold it in half and then in quarters, and place it on the scale.
  2. Tare the scale by pressing the Φ/T button so that the scale reads 0.0 g with the paper on it. See Figure 16.

A photo of a folded piece of paper atop a digital scale. The display on the scale reads 0.00 grams.
Figure 16.

Taring paper on a digital scale.

  1. Place 8.0 grams of sugar on the paper. Record the mass in Data Table 8.
  2. Carefully transfer the sugar from the white paper into the volumetric flask.
  3. Add approximately 15 mL of distilled water to the volumetric flask containing the 8.0 g of sugar, and swirl the flask until the sugar is completely dissolved in the water.
  4. Once the sugar is dissolved, use the short stem pipet to add additional water to the 25 mL mark. See Figure 17.

A photo of a researcher inserting a pipet into the neck of a volumetric flask.
Figure 17.

Adding water to a volumetric flask.

  1. Record the total volume in Data Table 8.
  2. Determine the concentration of the sugar solution in the volumetric flask using the following equation:

%m/v=Mass of Sugar (g)Total Volume (mL)×100%%m/v=Mass of Sugar (g)Total Volume (mL)×100%

  1. Record the concentration of the sugar solution in three places: In Data Table 8, for “Concentration,” in Data Table 9 for “Final Concentration” in the “Solution 0” row, and in Data Table 9 under “Initial Concentration” in the “Solution 1” row.
  2. Tare the scale by pressing the Φ/T button so that the scale reads 0.0 g.
  3. Place the volumetric flask containing the solution on the scale.
  4. Subtract the mass of the dry, empty volumetric flask from the mass of the flask containing the solution. The difference is the mass of the sugar solution.
  5. Record the mass of the sugar solution in Data Table 9 for “Solution 0.”
  6. Calculate the density of the sugar solution and record it in Data Table 9 for “Solution 0.”
  7. Pour the sugar solution (Solution 0) into the dry, empty 100 mL glass beaker.
  8. Rinse the volumetric flask well with distilled water.
  9. Use the serological (graduated) pipet to transfer 2.5 mL of Solution 0 into the volumetric flask.
  10. Add approximately 15 mL of distilled water to the volumetric flask containing Solution 0 and swirl to thoroughly mix.
  11. Using the short stemmed pipet, continue adding distilled water to the 25 mL mark of the flask.
  12. Tare the scale by pressing the Φ/T button so that the scale reads 0.0 g and then place the volumetric flask containing the solution on the scale.
  13. Subtract the mass of the dry, empty volumetric flask from the mass of the flask containing the solution.
  14. Record the mass of the new sugar solution in Data Table 9 for “Solution 1.”
  15. Calculate the density of the sugar solution and record in Data Table 9 for “Solution 1.”
  16. Determine the Final Concentration (% m/V) for Solution 1 using the following equation:

C1×V1=C2×V2In this equation:C1=Initial Concentration of Solution 0,V1=Volume Transferred,V2=25 mL,C2=Final Concentration of Solution 1C1×V1=C2×V2In this equation:C1=Initial Concentration of Solution 0,V1=Volume Transferred,V2=25 mL,C2=Final Concentration of Solution 1

  1. Record the Final Concentration of “Solution 1” in Data Table 9 under “Final Concentration.”
  2. Keep Solution 0 for the remainder of the experiment
  3. Pour Solution 1 from the volumetric flask down the drain.
  4. Repeat steps 20-31 for the remaining 3 dilutions using Solution 0 (4.5 mL, 3.0 mL, and 6.0 mL) in Data Table 9.

Note: In step 21, transfer the appropriate volume of sugar solutions (4.5 mL, 3.0 mL, and 6.0 mL). Use the concentration of solution to determine the concentration after dilusion.

  1. Create a graph displaying the relationship between Concentration and Density for the sugar solution. The x-axis on the chart will be Concentration and the y-axis is Density. Upload an image of the graph into Graph 1.

Prof. Angela

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