A Biotech Company Immune

1. A biotech company ImmunX, has obtained blood samples from 2 groups of human volunteers (with their permission/consent of course). It appears that 10 of these people have melanoma and 10 do not. The 10 who are currently cancer patients have a family history of cancer, whereas the other 10 healthy human volunteers have no family history of any cancer. You are hired by the company to help identify the genes (in the healthy subjects) that may be responsible for protecting the human volunteers from developing cancer and/or identifying the genes (in the cancer patients) that contribute to the disease. Explain what techniques you could use (and why) to help identify differentially expressed genes in the two groups of people. Also, if you suspect that mutations exist in the DNA that are inherited within the families that contribute to disease, what DNA sequencing techniques would you use (and why) to address this?

Prof. Angela


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